Micropropagation of Damask rose cvs Ghamsar and Azaran
Nikbakht, A., M. Kafi, M. Mirmasoumi and M. Babalar. 2005. Micropropagation of Damask rose cvs Ghamsar and Azaran. International Journal of Agriculture and Biology. 7 (4) :535-538
This research was performed to investigate the regeneration of two Iranian cultivars of Damask rose (Rosa damascena Mill.), "Azaran" and "Ghamsar" in vitro. The shoot single node segments included lateral buds were taken from bushes. After external disinfections for internal (bacterial) disinfections, appropriate antibiotics for each cultivar were used. Research findings showed that among 12 different media, a liquid modified MS (Murashige & Skoog) medium (with eliminated Cl- and reduced NH4+ ions) caused the best growth of newly proliferated shoots and no senescence occurred. BA (Benzyl Adenine) in four levels (0, 1, 2 and 3 mg L-1), GA3 (Gibberellic Acid) in four levels (0, 0.1, 0.25 and 0.5 mg L-1) and NAA (Naphthalene Acetic Acid) in two levels (0 and 1 mg L-1) were used and 32 different combinations of them were tested to investigate the most suitable hormonal treatment for establishment and multiplication stages as a factorial test on the base of a completely randomized design. Finally it was shown BA (1-2 mg L-1), GA3 (0.1 mg L-1) and NAA (0-0.1 mg L-1) for "Azaran" and the same concentrations of BA and GA3 but with 0 mg L-1 NAA for "Ghamsar" had the best proliferation and multiplication rate, appearance and leaf color. For in vitro rooting, quick deep treatment of microshoots' bottom in 2000 ppm IBA (Indole Butyric Acid) solution and then rooting in liquid half strength of the same MS medium showed the best result compared with 1000 ppm.